Protein Expression, Purification and Analysis
Lectures cover chemical properties of proteins, protein folding, solubility, charge, structure, posttranslational modifications; protein synthesis, recombinant protein expression including cloning strategies, expression plasmids, expression systems; chromatography techniques for protein purification. Laboratory work involve making gels and SDS-PAGE electrophoresis, purification of native proteins with ion exchange and salting out technique; purification of GST tagged proteins on glutathione agarose column and His-tagged proteins on Ni-NTA column; measuring of protein concentration and assays for protein activity; Western blot.
Prerequisites: Bio 201 and Ch 340 or permission of instructor.
Course Code: Bio 422