Cooper Union

Protein Expression, Purification and Analysis

Lectures cover chemical properties of proteins, protein folding, solubility, charge, structure, posttranslational modifications; protein synthesis, recombinant protein expression including cloning strategies, expression plasmids, expression systems; chromatography techniques for protein purification. Laboratory work involve making gels and SDS-PAGE electrophoresis, purification of native proteins with ion exchange and salting out technique; purification of GST tagged proteins on glutathione agarose column and His-tagged proteins on Ni-NTA column; measuring of protein concentration and assays for protein activity; Western blot.

Prerequisites: Bio 201, Ch 110, and Ch 111

Course Code: Bio 422

Founded by inventor, industrialist and philanthropist Peter Cooper in 1859, The Cooper Union for the Advancement of Science and Art offers education in art, architecture and engineering, as well as courses in the humanities and social sciences.
“My feelings, my desires, my hopes, embrace humanity throughout the world,” Peter Cooper proclaimed in a speech in 1853. He looked forward to a time when, “knowledge shall cover the earth as waters cover the great deep.”
From its beginnings, Cooper Union was a unique institution, dedicated to founder Peter Cooper's proposition that education is the key not only to personal prosperity but to civic virtue and harmony.
Peter Cooper wanted his graduates to acquire the technical mastery and entrepreneurial skills, enrich their intellects and spark their creativity, and develop a sense of social justice that would translate into action.